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UNIVERSITATEADETIINEAGRICOLE

IMEDICIN VETERINAR

IONIONESCUDELABRADIAI

LUCRRITIINIFICEVOL.53(12)

MEDICIN VETERINARPARTEA2

EDITURAIONIONESCUDELABRAD

IAI2010

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COLEGIULDEREDACIE

Redactorresponsabil:

Prof.univ.dr.GheorgheSOLCAN

Redactoradjunct:

Prof.univ.dr.OctavianZaharieOPREAN

Membri:

Prof.univ. dr.CorneliuCOTEA

Prof.univ.dr.VasileVULPE

Prof.univ.dr.MihaiCARPCRARE

Prof.univ.dr.DanDRUGOCIU

COMISIADEREFERENITIINIFICI

Prof.univ.dr.OctavianZaharieOPREAN

Prof.univ.dr.AbdelfatahNOURUniversitatea Pudue,SUA

Prof.univ.dr.H.C.Francois CRESPEAUENVAlfort,France

Prof.univ.dr.GheorgheSOLCAN

Prof.univ.dr.LiviuMIRON

Prof.univ.dr.GheorgheSVUAProf.univ.dr.GabrielPREDOIFMVBucureti

Prof.univ.dr.IoantefanGROZAFMVClujNapoca

Prof.univ.dr.GheorgheDRBU FMVTimioara

Prof.univ.dr.CorneliuCOTEA

Prof.univ.dr.MihaiCARPCRARE

Conf.univ.dr.erbanMOROANINSERMParis

Prof.univ.dr.H.C.LiviuRUNCEANU

Prof.univ.dr.TudorPERIANU

Prof.univ.dr.IoanCOMAN

Prof.univ.dr.ElenaVELESCU

Volumulafosteditatcusprijinulfinanciaral

MinisteruluiEducaiei,Cercetrii,TineretuluiiSportului

ISSN:14547406

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CURPINS

PARTEAI

ALINAANTON,GHEORGHESOLCAN,VASILEBOGHIAN,NICOLAEHAGIUBIOCHEMICALANDHAEMATOLOGICALPROFILEINTHEADVANCEDGESTATION

PERIODOFCOPPERDEFICIENTHOLSTEINANDBROWNSWISSCATTLE

ATTIA,H.FANDMAZHER,KHISTOLOGICALANDIMMUNOHISTOCHEMICALSTUDIESOFTHEBUCK'SPINEAL

GLANDDURING

LIGHT

AND

DARK

PERIODS

S.BESCHEACHIRIACCOMPARATIVESTUDYOFVASCULARARTERIALREACTIVITYATSEVERALMAMMAL

SPECIES: .THEREACTIVITYOFARTERIALSMOOTHMUSCLESATTHE

VASOCONSTRICTORAGENTSANTIDIURETICHORMONE(VASOPRESSIN)

BOGHIANV.,MLNCU R.N.,ACATRINEID.,PACAS.,ANTONALINA,SOLCANGH.MORPHOCLINICALASPECTSOFBABESIOSISINHORSES

IOANABURCOVEANU,I.BURTAN,ROXANATOPAL,L.C.BURTAN,M.FNTNARIUKERATOPATHIESINCARNIVORES:CLINICALSIGNSANDLOCALPATHOLOGIC

RESPONSES

HLNEHUET ,DELPHINEFRANKO ,CHAKIBDJEDIAT,EVAPEREZ,FRANOISCRESPEAU ,AMAURYDELUZE

PATHOLOGICALEFFECTSANDTISSUEDISTRIBUTIONOFMICROCYSTINLR(MCLR)

AFTER48HOURSNONINVASIVEEXPOSITIONOFNEWLYHATCHEDMEDAKA

(ORYZIASLATIPES)ELEUTHEROEMBRYOS

GH.DRBU

,V.COZMA

,K.IMRE

,A.BEJAN

,M.S.ILIE

,MIRELAIMRE

PREVALENCEOFCRYPTOSPORIDIUMSPP.ANDOTHERENTEROPATHOGENS

INFECTIONSATCALVESINWESTERN,CENTRALANDNORTHWESTERNROMANIA

GALA.F.,CATOIC.,BABAAI,MICLAUSV.,BOLFAP., TAULESCUM,TABARANF.,NAGYA.,MOUSSAR.,COSMINACUCASPECTSREGARDINGVASCULOGENICMIMICRYINCANINEMAMMARYCANCER

GRECUMARIANA,NSTAS V.,MAREM.,MORARURAMONA,HRICULUMINIADIANA,ILIECORNELIA

ASSESSMENTOFTHEANTIINFLAMMATORYACTIONOFTHECARPROFENBETA

CYCLODEXTRINSCOMPLEX

ON

EXPERIMENTAL

INFLAMMATION

MODEL

IN

RATS

7

13

20

26

30

36

44

49

60

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GROZAI.,GROZADARIA,PALLEMOKE,CENARIUM.,CIUPESIMONA,LAURAPARLAPANEVALUATIONOFDEGREEOFENGRAFTMENTINMOUSEMODELOFSTEMCELLS

HARVESTEDFROMHUMANPLACENTA

IONELAHOTEA,GH.DARABUS,C.PACURAR,TATIANARUGEA,P.MUNTEAN,M.S.ILIE,K.IMRE,MIRELAIMRE,DENISASORESCU,ADRIANBALINT,DINUINDREPRELIMINARYSTUDYONTHEPREVALENCEOFTOXOPLASMAGONDIIINFECTIONIN

WILDBOARSFROMTIMISCOUNTY

OLIMPIAC.IACOB, B.C.CEPIDEMIOLOGICALINVESTIGATIONSONDIGESTIVEPARASITOSIS INRACINGPIGEONS

ANDTHERISKOFRELEASINGPARASITICELEMENTSINFREEAREAS

MARIANAIONITA ,D.K.HOWE,I.L.MITREA,B.STEVENSON,MICHELLEYEARGAN

PRELIMINARYDATACONCERNINGOPTIMIZATIONOFAPCRBASEDMETHODFOR

MOLECULARDETECTION

OF

TICK

BORNE

PATHOGENS

ISMAIL,S.F;ABDALGALIL,A.S.AANDGEHAN,B.A.YOUSSEFPROPOFOLANAESTHESIAINDONKEYSINCOMBINATIONWITHCHLORALHYDRATE

ADINAMARIAMANEA,S.E.GEORGESCU,STELIANAKEVORKIAN,SORINADINESCU,MARIETACOSTACHEGENOTYPINGESTROGENRECEPTORPOLYMORPHISMINPIGS,USINGTHEPCRRFLP

METHOD

MICLU V.,ANNECLAUDIATEFNU,ADRIANAMUREAN, C.OBER,V.RUS

COMPARATIVETESTINGOFSOMEEXPERIMENTALMODELSOFOXYGENINDUCED

RETINOPATHYINYOUNGRATS.HISTOLOGICALSTUDY.

MOUSSARAOUAD.,CCATOI.,BSEVASTRE.,MTAULESCU.,PBOLF.,AGAL.,,F.ATABARAN.,A.LNAGY.,CCUC.EXPRESSIONOFTHEVIMENTINMARKERINDOGMELANICCUTANEOUSTUMORS

S.OANCEA,G.PAVEL,A.V.OANCEA

ONTHESHAPEOFTHEERYTHROCYTESFROMSOMEHERBIVOREMAMMALS

S.OANCEA

,S.PADUREANU

,A.V.OANCEA

IDENTIFICATIONOFPATHOLOGICALSTATESBASEDONREDBLOODCELL

AGGREGATION

OPREANO.Z. ,GHEBANDIANA ,FORNANORINACONSUELA,INDILARE.V.,GRMAD S.

STRUCTURALMODIFICATIONSOFTHEORALMUCOSAANDTHEDENTALAPPARATUS

INDUCEDBY SOMEDRUGSINLABORATORYMICE

EMOKEPALL,GROZAI.,CENARIUM.,CRISTINAILEA,OLGASORITAU,CIPRIANT.,BERCEC.ISOLATION,CHARACTERIZATION,PHENOTYPIZATIONANDDIFFERENTIATIONOFSTEM

CELLSFROMRATPLACENTA

65

70

74

87

96

103

107

115

121

127

131

141

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DUMITRIARUGINA,ADELAPINTEA,ANDREABUNEA,RALUCAPOP,SANDAANDREILUTEINPREVENTSHIGHGLUCOSEINDUCEDOXIDATIVESTRESSINHUMANRPECELLS

TRIFALEXANDRA

,DUMITRESCUEUGENIA ,PETROVICISNEJANA

ALUMINIUMSULPHATEIMPACTONFUNDAMENTALBIOMARKERSOFREPRODUCTIVE

FUNCTIONALITYINFEMALERATS(SUCKLINGPERIODEXPOSURE)

WAELM.ELDEEB,S.M.ELBAHRIMPROVEMENTOFGLUCOSECONCENTRATION,LIPOPROTEINPROFILEAND

ANTIOXIDANTBIOMARKERSINBLOODOFNATURALLYDIABETICBITCHES

ADMINISTEREDINSULINWITHVITAMINCORVITAMINE

WAELM.ELDEEB, ABDELAZIZALMUJALLI, S.M.ELBAHR

INVESTIGATIONOFSELECTEDBIOCHEMICALINDICATORSOFEXERTIONAL

HABDOMYOLYSISIN

ARABIAN

HORSES:

PRO

INFLAMMATORY

CYTOKINES

AND

OXIDATIVESTRESSMARKERS

IHABEL_ZOGHBY,AHMEDKASSAB

THEPARSDISTALIS(ANTERIORPITUITARY)INONEHUMPEDCAMEL(CAMELUS

DROMEDARIUS ):AMORPHOLOGICALSTUDY

IHABM.ELZOGHBYLIGHTANDELECTRONMICROSCOPESTUDIESOFTHEADRENALGLANDSOFTHE

EGYPTIANGEESE(ALOPOCHENAEGYPTIACUS)

PARTEAII

GEHANSAIDAHMEDAFIFYDETERMINATIONOFSOMEANTIMICROBIALRESIDUESINCHICKENMEATAND

GIBLETS.

ADRIANAANI,DRAGO ANI,GHEORGHESAVUARESEARCHESREGARDINGTHESEROPREVALENCEOFSWINEHEPATITISEVIRUS

INFECTIONIN

THE

EAST

OF

ROMANIA

DRAGO CONSTANTINANI,ADRIANAANI,GHEORGHESAVUASEROEPIDEMIOLOGICALSTUDYREGARDINGINFECTIOUSBOVINERHINOTRACHEITIS

INCOUNTIESFROMNORTHOFMOLDOVAREGION

CRISTINABULBAA(PANAITE),D.DRUGOCIU,DANADRUGOCIU,PANAITEC.G.USINGSOMATICCELLSCOUNTANDBACTERIALCOUNTTOEVALUATEMILK

PRODUCTIONINONEDAIRYFARMINIASSYCOUNTRY

PRIMIANIEDIANINGSIH,JANALEXSIWISELECTION

RESPONSES

OF

ALABIO

DUCK

(ANAS

PLATIRINCHOS

BORNEO)

PRODUCTIONININTENSIVEMAINTENANCESYSTEM

145

153

158

170

183

195

213

219

222

226

230

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EL KOMY.A.A.A,ELZOGHBY.R.R, ABDELAZEM.M.A.TERATOLOGICALANDPATHOLOGICALSTUDIESOFCEFOPERAZONEINFEMALE

ALBINORATS

SERGIUEMILGEORGESCU,MARIAADINAMANEA,STELIANAKEVORKIAN,MARIETACOSTACHE

ANEWMETHODFORANALYZINGTHEAGOUTILOCUSINVOLVEDINTHECOATCOLOUROFHORSES

CRISTINAHORHOGEA,IVONALAIU,CRISTINARMBU,MIHAICARPCRAREFELINEINFECTIOUSPERITONITISCASEPRESENTATION

STELIANAELVIRAMARIAKEVORKIAN,S.E.GEORGESCU,MARIAADINAMANEA,MARIAGEORGIANAGAVRILA,G.HRINCA,MARIETACOSTACHETHESPIDERLAMBSYNDROMEABSENCEINFIVEROMANIANSHEEPBREEDS

HENDRONOTOARNOLDUSWALEWANGKOLENGKEY,LOVITAADRIANI

IMPLICATIONEFFECTOFPROBIOTICBACTERIATOYOGHURTQUALITYANDENZYME

ACTIVITIES

LOVITAADRIANI,HENDRONOTOA.W.LENGKEYPROBIOTICBACTERIAASYOGHURTSTARTERANDITSIMPLICATIONEFFECTTOTHE

PATHOGENICANDNONPATHOGENICBACTERIAINMICEGASTROINTESTINAL

INAIULIANAMACOVEI,S.MANOLESCU,CRISTINARMBU,S.PASCA,G.DRAGAN,GH.SAVUASTUDYOFANOUTBREAKOFFOWLTYPHOID INPHEASANTS

CRISTINARMBU,ELEONORAGUGUIANU,GH.SOLCAN,CRISTINAHORHOGEA,E.V.INDILAR,CTIN.PAVLI,C.CARPCRARECONSIDERATIONSONTHEASSOCIATIONOFPERIODONTALDISEASEWITHOTHER

ORGANICDISEASESINDOGSANDCATS

ROOSTITAL.B.,CISSYR.P.,ERICF.S.,SRIM.,HENDRONOTOA.W.L.THEINFLUENCEOFSEASONALCHANGETOWARDSTHENUMBEROFOUTBREAKSAND

POULTRYDEATHRATECAUSEDBYAVIANINFLUENZAATBANDUNGDISTRICT

GH.SAVUA,IULIANAONI,ADRIANAANI,D.ANI,LUANDALUDU,BEJANARIUANAEPIDEMIOLOGICALINVESTIGATIONSINTHEEASTOFROMANIAREGARDINGTHE

SEROPREVALENCEOFINFLUENTZAATYPEVIRUSESINDIFFERENTSPECIESOF

ANIMALS

JANALEX SIWI,PRIMIANIEDIANINGSIHANDDUDUNGMULLIADIPERFORMANSGENETICQUALITATIVEANDQUANTITATIVEOFTHINTAILSHEEPAND

PRIANGANSHEEP

N.STARCIUC.,NATALIAOSADCI.,I.SCUTARU.,T.SPATARU.

THEOUTBREAKS

OF

INFECTIOUS

BRONCHITIS

OF

CHICKENS

IN

PRIVATE

POULTRY

FARM

236

246

249

254

259

262

267

271

278

282

286

294

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OANA RALUCASTRUGARU,FILIPPO TURRINI,ALESSANDRASCAGLIARINI,ELENAVELESCUTHEDETECTIONOFORFVIRUSBYPCRATTHERUMINANSOFROMANIA

ALINAVLAD

SABIE,

VIOREL

FLORI

TEAN,

CARMEN

CRE

U,

MIHAI

OBAD

,CTLIN

CARP CRARE,MIHAICARP CRAREDETECTIONANDQUANTIFICATIONOFSALMONELLASPP.ANDCAMPYLOBACTER

JEJUNIONPOULTRYCARCASSES

BYREALTIMEPCR

MOHAMEDYOUSEFRAMADANANDABLADESOKYABDELMAGEIDEPIDEMIOLOGICALSTUDYOFECTOPARASITESINSTRAYDOGSIN KALUBYIA

GOVERNORATEOFEGYPTWITHASPECIALREFERENCETOITSCONTROLINPUPPIES

BYDELTAMETHRINANDIVERMECTIN

MARIAZAMORNEA,D.ERHAN,.RUSU,NINATLMBU,O.CHIHAI,VIORICACOADESTIMATIONOFVEGETALEXTRACTSEFFICIENCYINDOMESTICBIRDS

ECTOPARASITOSESTREATMENTANDPROPHYLAXIS

297

300

307

318

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DETERMINATIONOF

SOME

ANTIMICROBIAL

RESIDUESINCHICKENMEATANDGIBLETS

GEHANSAIDAHMEDAFIFY

Dept.FoodHygieneandControl,Fac.Vet.Med.,BenhaUniversity,Egypt.

e.mail.drgehan222@yahoo.com.

SUMMARY

Forty five samples of muscle, gizzard and liver (15 of each) were collected from poultry shops inKalyobiagovernorateandexaminedforthepresenceoftetracyclineandsulflaquinoxalineusingfour

platetest(FPT)andhighperformance liquidchromatography(HPLC) .Also,theeffectofboilingasa

methodofcookingontheseresidues wasstudied.

The results showed that 13.33%, 13.33% and 26.67% of muscle, gizzard and liver samples were

positiveforthepresenceoftetracyclineresiduesbyFPTmethod,respectively,however,13.33%,20%

and 40% were positive for such residues by HPLC method, respectively. Moreover, non of the

examinedmusclesampleswereexceededthemaximumpermissiblelimitoftetracyclinewhile,6.67%

and20%oftheexaminedgizzardand liversampleswereexceeded,the maximumpermissible limit,

respectively.Concerning,sulfaquinoxalineresidue,itwasfoundthat20%,26.67%and33.33% ofthe

muscle,gizzardandliversampleswerepositivebyFPTmethod,while26.67%,33.33%and46.67%by

HPLC, respectively. Accurately , 33.33% , 20% and 40% of the examined muscle, gizzard and liver

sampleswere exceededthemaximumpermissiblelimitofsulfaquinoxaline,respectively.Onboiling , the levelsof tetracycline residues werereduced by 100%formuscle sampleswhile this

percentage reached to 82.35% and 79.67% reduction for the examined gizzard and liver samples,

respectively. Also, the highest reduction% for Sulfaquinoxaline residues was 100 for muscle sample

after boiling, while 78.18% and 73.04% reduction were shown in gizzard and liver samples,

respectively.Thepublichealthsignificanceofsuchantimicrobialagentsandsomerecommendations

tocontroltheirpresenceinchickenmeatandgibletswerediscussed.

Introduction

Chickenmeat,derivedmainlyfrombroilerisconsideredasthefavoritefoodforEgyptiansdueto its

relatively low price n relation to the red meat. A large number of drug used to control or prevent

infectionsortopromotegrowthareessentialinmodernpoultry

productionsystem.Antibioticsandsulfonamidesarethe twooftheoldestgroupsusedinveterinary

medicine.(National

Registration

Authority

for

Agricultural

and

veterinary

chemicals,

2000).

Antibiotics are widely used in poultry farms as a dietary supplements. Tetracycline is one of these

antibioticswhichhasabroadspectrumactiononavarietyofinfection.(SchenckandCallery,1998).

About60%ofaningesteddoseoftetracyclinewasabsorbedfromthegastrointestinaltractofhuman

beings andthenwidelydistributedinthebody,particularlyliver,kidney,bonesandteeth.However,

theuseofthiscompoundmayresultinresiduesinpoultrymeatespeciallyifproperwithdrawaltimes

havenotbeenused(Czeizeletal.,1998).

Thesecondcommonlyuseddruginchickenbroilersissulfaquinoxalinewhichusedforthetreatment

ofcoccidiosisandawiderangeofbacterialdisease.Producersmay beunwillingtowithholdthedrug

forasufficientlongperiodtoensurethatallresidueshavebeeneliminatedbeforeslaughter,sothe

residue of sulfaquinoxaline may be retained in the edible tissues of marketed chicken broiler (

Kozarovaetal.,2001aandkozarovaetal.,2001b)and(Sutiaketal.,2000).

Sulfaquinoxalineisalsoadministeredtopoultryatarateinexcessofthatrecommendedoratmore

frequent interval than specified. Therefore, other negative effects of sulfonamide on human body

213

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UniversitateadetiineAgricoleiMedicin Veterinar Iai

+

-

+

-+

-

+

-

+

-

+

-

+

-

RESULTS

Table(1)Incidenceandlevelsoftetracyclineresidues(ug/ kg)intheexaminedsamplesofbroiler

muscleandgiblets(n=15).

Methodofdetection

Examined samples

PositiveSamples Min Max Mean S.E

FPT * HPLC**

No % No %

Muscle 2 13.33 2 13.33 20 85 52.50 9.42++

Gizzard 2 13.33 3 20.00 35 340 168.23 26.81

Liver 4 26.67 6 40.00 65 910 316.67 35.09

*=FourPlateTest.

**=HighPerformanceLiquidChromatography.

++=Highsignificantdifferences(P

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Lucrritiinificevol53seriaMedicin Veterinar

Table(5)Effectofboilingontheantimicrobialresiduesintheexaminedsamplesofbroilermuscle

andgiblets.

Tetracycline(ug/kg) Sulfaquinoxaline(mg/kg)

Beforeboiling

Afterboiling Reduction%

Beforeboiling

Afterboiling

Reduction%

Muscle 85 ND 100 0.46 ND 100

Gizzard 340 60 82.35 1.10 0.24 78.18

Liver 910 185 79.67 3.45 0.93 73.04

ND=NotDetected

DISCUSSION

Residues of veterinary drugs in food of animal origin represent a risks to human health

and they have negative impact on the technological processes in the food industry. Accordingly,the improper use of veterinary drugs may result in drug residue in the poultry tissues causing

allergicreactionsinsensitiveindividualsconsumingsuchfoodstuff(Kozarovaetal.,2001a,b).

Resultsachievedin table(1)declaredthat13.33%,13.33%and26.67%oftheexaminedsamples

of broiler muscle, gizzard and liver were positive for the presence of tetracycline residue by

applicationof FPTtrial,respectively,however, suchpercentages were13.33%, 20% and 40% by

using HPLC, respectively. Thus, there was awide variation between the two methods of

tetracyclinedeterminationGenerally,theleveloftetracyclineresiduewererangedfrom20to85

with an average of 52.50 9.42 ug /kg for broiler muscle, 35 to 340 with an average of 168.23

26.81ug/kgforgizzardand65to910withan averageof316.67ug/kgforliver.Thedifferences

betweentheexaminedsamplesofbroilermuscleandgibletswerehighlysignificant(P