SpeciiSpecii din genul din genul Actinobacillus si HaemophilusActinobacillus si Haemophilus

cu semnificatie patologica cu semnificatie patologica pentru porcpentru porc

Dr. COSTIN STOICA

www.tgw1916.net

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FAMILIA FAMILIA PASTEURELLACEAEPASTEURELLACEAE

PASTEURELLA ACTINOBACILLUS HAEMOPHILUS

GENURI:

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Dendrograma privind relatiile filogenetice ale unor reprezentanti ai familiei Pasteurellaceae pe baza similaritatii secventei ARNr 16S

A. actinomycetemcomitans

H. aphrophilus H. segnis

H. haemolyticusH. influenzaeH. aegyptius

A. equuliA. suis

A. ureaeA. pleuropneumoniaeA. lignieresii

A. minorH. minor groupA. porcinus

A. indolicusH. parasuis (NCTC 4557)H. parasuis (strain Nagasaki)

Pasteurella species AH. taxon C P. gallinarum H.

paragallinarum

GENUL ACTINOBACILLUSGENUL ACTINOBACILLUSA. actinomycetemcomitansA. lignieresiiA. equuliA. seminisA. capsulatusA. pleuropneumoniaeA. minorA. porcinusA. indolicusA. suisA. ureaeA. rossiiA. murisA. hominis

( Bergey’s manual of Sistematic Bacteriology, 1994; Moller et al, 1996) REGNVM PROKARYOTAE

GENUL ACTINOBACILLUSGENUL ACTINOBACILLUSA. actinomycetemcomitansA. lignieresiiA. equuliA. seminisA. capsulatusA. pleuropneumoniaeA. minorA. porcinusA. indolicusA. suisA. ureaeA. rossiiA. murisA. hominis

( Bergey’s manual of Sistematic Bacteriology, 1994; Moller et al, 1996)

Specii din genul Actinobacillus Specii din genul Actinobacillus cu importanta pentru porccu importanta pentru porc

A. pleuropneumoniaeA. minorA. porcinusA. indolicusA. suis

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Serotipul de A. pleuropneumoniae Toxina 1 2 3 4 5a 5b 6 7 8 9 10 11 12 Apx I Apx II Apx III

Toxinele sintetizate de fiecare serotip de A. pleuropneumoniae

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Actinobacillus suis – coloratie Gram

Caractere biochimice diferentiale intre Caractere biochimice diferentiale intre

reprezentanti ai genului Actinobacillusreprezentanti ai genului Actinobacillus Caractere biochimice

A. minor

A. porcinus

A. indolicus

A. pleuro- pneumoniae

A. suis

Factor V + d + + - Hemoliza - - - d + CAMP - - - + Ureaza + - - + + Indol - - + - - Catalaza - - + d + ONPG + + + + + fucosidaza - d d - Arabinoza - d - - + Rafinoza + d + - + Lactoza + d d - + Manitol - d - + -

+ : >90% tulpini pozitive, - : > 90% tulpini negative, d : 11- 89% tulpini pozitive (Moler et al. , 1996)

GENUL HAEMOPHILUSGENUL HAEMOPHILUSH. parasuisH. taxon CH. taxon DH. taxon EH. taxon FH. ‘minor group’H. aegyptiusH. influenzaeH. aphrophilusH. haemolyticusH. segnisH. paragallinarum

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GENUL HAEMOPHILUSGENUL HAEMOPHILUSH. parasuisH. taxon CH. taxon DH. taxon EH. taxon FH. ‘minor group’H. aegyptiusH. influenzaeH. aphrophilusH. haemolyticusH. segnisH. paragallinarum

Specii din genul Haemophilus Specii din genul Haemophilus cu importanta pentru porccu importanta pentru porc

H. parasuisH. taxon CH. taxon DH. taxon EH. taxon DH. minor group

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Haemophilus parasuis – coloratie Gram

Caractere biochimice diferentiale intre Caractere biochimice diferentiale intre reprezentanti ai genului Haemophilusreprezentanti ai genului Haemophilus

Caracterebiochimice

H.minor group

H.parasuis

Taxon C Taxon D Taxon E Taxon F

Necesar factor V 100 100 100 100 60 100

Necesar factor X 0 0 0 0 0 0

Indol 0 0 0 0 0 100

Ureaza 100 0 0 0 0 0

Oxidaza 13 100 100 0 0 100

galactozidaza 100 100 0 100 80 100

fucosidaza 0 96 0 68 80 46

glucozidaza 70 4 100 64 20 89

Neuraminidaza 100 100 100 91 60 100

Red. nitratilor 100 100 100 96 30 100

Caractere biochimice diferentiale intre reprezentanti Caractere biochimice diferentiale intre reprezentanti ai genului Haemophilus (continuare)ai genului Haemophilus (continuare)

Caracterebiochimice

H.minor group

H.parasuis

Taxon C Taxon D Taxon E Taxon F

Arabinoza 0 0 100 86 0 0

Lactoza 91 0 0 96 0 32

Maltoza 100 100 100 100 20 100

Melibioza 78 0 0 96 0 18

Sucroza 100 100 100 100 10 100

Trehaloza 78 0 0 55 0 79

Rafinoza 100 0 100 100 0 93

Manitol 0 0 0 100 0 0

Sorbitol 0 0 0 100 0 0

Inozitol 0 4 100 77 0 0

BIBLIOGRAFIE:Andrew N. Rycroft, Lisa H. Garside, " Actinobacillus Species and their Role in

Animal Disease", The Veterinary Journal , 2000, 159, 18-36 Cristian Moller et al., ‘V Factor Dependent Members of the Family

Pasteurellaceae in the Porcine Upper Respiratory Tract’, Journal of Clinical Microbiology, Dec 1990, p. 2711-2716

Durda Slavic et al. " Prevalence of O1/K1 and O2/K3 Reactive A. suis in Healthy and Diseased Swine", Journal of Clinical Microbiology, Oct 2000, p. 3759-3762

Durda Slavic et al. "Comparative pathogenicity of different Actinobacillus suis O/K serotypes", The Canadian Journal of Veterinary Research, 81-86

J. Van Ostaaijen et al., " A. suis Strains Isolated from Healthy and Diseased Swine Are Clonal and Carry apxICABDvar suis and apxIICAvar suis Toxin Genes", Journal of Clinical Bacteriology

John G. Holt et al., “Bergey’s Manual of Determinative Bacteriology 9th Edition, 1994

Moller K. et al., Oct. 1996, "A. minor sp. nov., A. porcinus sp. nov., and A. indolicus sp. nov., Three New V Factor-Dependent Species from the Respiratory Tract of Pigs", International Journal os Systematic Bacteriology , Oct. 1996, 1131-1137  Radu Moga M@nzat et al., “Boli infec\ioase ale animalelor – bacterioze”, p. 157-188, Ed. Brumar 2001

Vicki J. Rapp-Gabrielson et al., "Prevalence of Haemophilus parasuis serovars among isolates from swine", Am. J. Vet. Res. Vol 53, No 5, 1992

THE ENDTHE END

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