protea ilmac 07 -cam

Protea’s 5,000 sq ft laboratory features BIOiTRAQ MALDI TOF/TOF (ABI 4800) and LTQ (Thermo) mass spectrometers

Isolate - Fractionate - Collect - Digest - Separate - Analyze

BIOLOGY clinical samples: ü  blood ü  tissue ü  urine ü  sputum

CHEMISTRY

Sample Analysis by

Mass Spec

Connecting Biology to Mass Spectrometry

Connecting Biology to Mass Spectrometry a new product offering

Products for Gel-Based Proteomics and Genomics

Products for Solution-Based Proteomics

Products for Capillary Electrophoresis

Services


BIOLOGY clinical samples: ü  blood ü  tissue ü  urine ü  sputum

CHEMISTRY

Sample Analysis by

Mass Spec

The GPR Gel Protein Recovery System

Ø Recovers intact proteins from gels

Ø Removes salts, detergents and dyes

Ø Improves sample quality for downstream MS

Ø Enables top-down proteomics

Ø Elutes proteins from 5kDa to 200 kDa

Ø Quantitative protein recovery data available


Related Gel-Based Proteomics Products

SDSaway detergent removal

Silver Staining Kit

Protein MW Markers

Solvates SDS detergent from the protein surface Fast, simple - works

Ultrafast Ultrasensitive (20-40ng/band)

Designed for use with Laemmli SDS-PAGE gels From 6,500 to 200,000 Da


Programmable Syringe Pump

Ø Fully programmable

Ø Operates standalone or from a computer

Accessories for Sample Preparation

ProteaGeltm Products

Ø Ready to use

Ø No stacking gel required = longer gel separations

Ø Stable at room temperature for one year

Ø Mass Spec Certified


SeaClear Agarose products

Ø Complete Line of agarose products

Ø Value Pricing

Ø Protea in-house Quality Testing


Proteases and ProteaBeadstm

Protea Endoproteinases Ø  Certified Mass Spec Grade Ø  Performance Tested

ProteaBeads Ø  Immobilized Trypsin & Glu-C

Ø  Rapid, efficient digestion

Ø Prevents contamination of sample with autodigestion products

Ø Available in flow-through cartridges


MALDI sample prep

Complete kit - optimizes samples for MALDI MS Removes Coomasie Stain bound to protein Uses ultrapure matrix


Figure 1 presents the effect of coomasie stain on the MALDI-TOF MS signal of a Myoglobin protein sample. The spectrum reveals different number of coomasie molecules bound to the protein molecules as indicated by the resolved adducts in the spectrum. This adduction of coomasie to protein molecules results in reduced sensitivity since the protein signal is spread over the different adducts. In addition, the coomasie-protein adducts can mask the presence of any post-translationally modified protein molecules. Removal of coomasie by destaining therefore results in an improved MS signal

UltraPure MALDI Matrices

Protea Ultrapure CHCA matrix Vs. competitor

Ø Improved MALDI-MS sensitivity

Ø Forms homogenous crystals

Ø Easy reconstitution


Reversed Phase Sample Preparation Products

Ø C18 ProteaTips - effective for salt removal

Ø Clean-up of peptides from in-gel digested proteins

Ø C18 ProteaPlate - 96 well sample cleanup

C4 & C8 Tips also available Supplied as kits with buffers and protocols


Titanium Dioxide Sample Preparation Products

For enrichment of phosphopeptides in protein digest samples, particularly complex protein/ peptide samples Provided as complete kits with sample reconstitution and wash buffers Also available in 96 well plates


ZICtm HILIC ProteaTips & Sample Prep Kit

Effective in removal of detergents & salts Enriches peptides complementary To those trapped by C18 ZipTips Low backpressure during Sample processing


Zic is a registered trade mark of Sequant AB

Facettm Columns for ESI Mass Spectrometry

Made of PEEK Biocompatible plastic Trapping Columns Now Available:

Reversed Phase (RP) Cation Exchange (CX) ZICtm -HILIC

Also available:

FACET microfilters


Zic is a registered trade mark of Sequant AB

Products for Capillary Surface Modification

Ø Capillary Coating Kit

Ø Reproducible control of EOF

Ø Stable for wide pH ranges

Ø Coatings resistant to irreversible Protein adsorption

Products for Capillary Electrophoresis

Plastic Chip Design & Fabrication Services

Features Plastic microfluidic chips 100 micron features Short turnaround times Applications Capillary electrophoresis Reaction monitoring Micro-sample prep

Services

Berkely.edu

Protein Research Services

Ø BIOiTRAQ capability

Ø Use of our new products, eg TiO2

Ø Gel-based sample capabilities

Ø De Novo Sequencing (PEAKS studio)

Services

www.appliedbiosystems

Protein Standards


Mark of Quality Assurance


gel protein recovery protein digestion protein separation Disposable micro-cartridges packed with protein separation matrices:

hydrophobic hydrophilic reverse phase ion exchange

Connecting Biology to Proteomics

protea ilmac 07 -cam

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