7/21/2019 Transfer Info Membranara

1/38

7/21/2019 Transfer Info Membranara

2/38

COMUNICAREA CELULAR

CELULELE COMUNIC !- Organism (1013-1014celule) rgane

esu"uri celule # sc$im%& 'ermanen"semnaleinrma"ii

-* e"a'e-sin"e+a mleculelr semnal-eli%erarea mleculelr semnal ,e c&"re celula care semnali+ea+&-"rans'r"ul semnalului c&"re celulele in"&-,e"ec"area semnalului ,e c&"re 'r"ein& rece'"are s'eciic&-r&s'uns celular.

-m,iicare a me"a%lismului celular sau a e/'resiei genice-m"ili"a"e celular&-'rlierare i ,ieren iere -mar"e celular& 'rgrama"& - a''"+&

-n,e'&r"area sau inac"iarea mleculei semnal 2i "erminarea r&s'unsuluicelulei in"&

7/21/2019 Transfer Info Membranara

3/38

RAN5UC6IA EMNALULUI # (en) signal "rans,uc"in

- ermen cnsacra" MEDLINE - 1972

-3 elemen"e-mlecule semnal-en,gene-e/gene (s"imuli am%ien"ali)

-rece'"ri-e/"racelulari

-in"racelulari-c&i ,e semnali+are-la ,is"an&

-en,crin&-neurcrin&-'aracrin&

-au"crin&-'rin cn"ac" ,irec"(7u/"acrin&)

-7nciuni celulare

7/21/2019 Transfer Info Membranara

4/38

RAN5UC6IA EMNALULUI # (en) signal "rans,uc"in

-M,ali"&i ,e r&s'uns

-ac"iare genic& # sin"e+& 2i secreie celular&

-ac"iareainac"iarea unei c&i me"a%lice

-'rlierareamar"ea celular&

-s"imulareain$i%area m"ili"&ii celulare

7/21/2019 Transfer Info Membranara

5/38

-en,gene-$rmni # e/8 mela"nin&-ac"ri ,e cre2"ere # ac"r ,e cre2"ere e'i,ermic& E9:-cm'nen"e MEC # i%rnec"in& :N-ci";ine # molecule semnal mici (proteine, peptide,

glicoproteine) exI:-gamma-c$em;ine # ci";ine c$em"ac"ice-neur"ransmi&"ri # ace"ilclin&-neur"rine # ac"r ,e cre2"ere neurnal&

-e/gene(s"imuli am%ien"ali)-s"imuli i+ici # ra,iaii-s"imuli c$imici-"/ine %ac"eriene-irusuri

Molecule semnal

7/21/2019 Transfer Info Membranara

6/38

-care nu "raersea+& 'lasmalema-lan

7/21/2019 Transfer Info Membranara

7/38

!eceptori

e/"racelulari

in"racelulari

RECE>ORI MEM?RANARI:R

ACI@IAECAALIIC

CUACI@IAECAALIIC

R

R>

CAN8ION

9>CR

CB-R

R9C

7/21/2019 Transfer Info Membranara

8/38

!eceptori

>e'"i,ul na"riure"ic a"rial (>NA). e/creia NaD mirela/are 8 ne"e,e asc8

>NA Rec >NA

-e/"racel. rec-in"racel. 9C 9>

9M>cF>

"rotI#

"rotI# >

- Domeniu I# catalitic -

R9CR cu ac"ii"a"e 9C (guanila"-cicla+ic&)R cu ac"ii"a"e 9C (guanila"-cicla+ic&)

7/21/2019 Transfer Info Membranara

9/38

!eceptoriRece'"ri "ir+in-;ina+ici# ac"ii"a"e ca"ali"ic& in"rinsec&

Rece'"ri18

9: G

F8

- Insulina- @E9:- >59:- E9:

- Domeniu I# catalitic -

R

7/21/2019 Transfer Info Membranara

10/38

!eceptoriRece'"ri "ir+in-;ina+ici# ac"ii"a"e ca"ali"ic& in"rinsec&

RRECE>ORI CU ACI@IAE ERIN-ORI CU ACI@IAE ERIN-

7/21/2019 Transfer Info Membranara

11/38

!eceptoriRece'"ri "ir+in-;ina+ici# ac"ii"a"e ca"ali"ic& in"rinsec&

R

- ! 2;dintre proteinelecelulelor eucariote

- #atali$ea$% trans&erul "i din

degradarea '" pe re$iduuri BRale proteinelor int%

- !L!I- Cn"rlul CC- Migrare celular&

- Me"a%lism celular

RECE>ORI CU ACI@IAE IROHIN-INAHICRECE>ORI CU ACI@IAE IROHIN-INAHIC

7/21/2019 Transfer Info Membranara

12/38

!eceptoriRece'"ri "ir+in-;ina+ici# ac"ii"a"e ca"ali"ic& in"rinsec&

RRECE>ORI CU ACI@IAE IROHIN-INAHICRECE>ORI CU ACI@IAE IROHIN-INAHIC

- 5meniu e/"racelularglico$ilat, receptea$% ligandul,

- 5meniu "ransm%uni+elix

- 5meniu ci"'lasma"iccca 1

7/21/2019 Transfer Info Membranara

13/38

!eceptoriRece'"ri "ir+in-;ina+ici# ac"ii"a"e ca"ali"ic& in"rinsec&

RRECE>ORI CU ACI@IAE IROHIN-INAHICRECE>ORI CU ACI@IAE IROHIN-INAHIC

- Dimeri$area !- 'uto-" re$ =! dom citosolice- =!-"cu'lea+& mlecule cu

,menii ?(p+osp+ot6rosine4inding)

- 'ceste proteine anga3ea$% altec%i de semnali$are

7/21/2019 Transfer Info Membranara

14/38

!eceptori

Rece'"ri "ir+in-sa"a+ici# ac"ii"a"e ca"ali"ic& in"rinsec&

>r"eina C54J leucci"ar&.

- Domeniu I# tiro$in &os&ata$ic . de&os&orilare a unor proteine I#

R>

7/21/2019 Transfer Info Membranara

15/38

Rece'"ri &r&ac"ii"a"e ca"ali"ic&

in"rinsecac"iarea c&ii KA-

A

Kanus-;ina$e-ignalransducer and

Acti0ator o&ranscription

RECE>ORI MEM?RANARI:R

ACI@IAECAALIIC

CUACI@IAECAALIIC

R

R>

CAN8ION

9C>R

CB-R

R9C

7/21/2019 Transfer Info Membranara

16/38

!eceptoriRece'"ri &r& ac"ii"a"e ca"ali"ic& in"rinsec ac"iarea c&ii KA-AKanus-;ina$%-ignal ransducer and Acti0ator o& ranscription

Rece'"ri('r"8 ransm%8 uni'ass).-inter&eroni,-interleu>ine (Il-2, ?, @, A, B, 7, 11),-&actor de creCtere (5:),-prolactina ("!L),-eritropoietina (E"),

-trom4opoietina,-&actor granulocitar-macro&agicstimulator de colonii 5M-#

-Nu pre$int% acti0itate catalitic%-e asocia$% cu " I#-!eceptorii dimeri$ea$% pentru acti0are-!%spuns mult mai rapid prin acti0areadirect% a transcripiei

7/21/2019 Transfer Info Membranara

17/38

#%i de semnali$are

La ,is"an&

Cn"ac" ,irec"

7/21/2019 Transfer Info Membranara

18/38

#%i de semnali$are

7/21/2019 Transfer Info Membranara

19/38

#%i de comunicare . la distan%

EN5OCRINE >ARACRINE

AUOCRINE NEUROCRINE

7/21/2019 Transfer Info Membranara

20/38

ransmiterea de in&ormaiiprin +ormoni polipeptidici

-Etap% mem4ranar%-Etap% citoplasmatic%

-Etap% mem4ranar%

-receptori-traductor - proteinele 5-ampli&icator ('#*5#)-mesager secund ('M"c, 5M"c, #a, I"?, D'5

-Etap% citoplasmatic%-mesager secund

-acti0area >ina$elor . serin*treonin >ina$e-trans&er% " pe re$id ''-er*+r

-&os&orilare * inacti0are &os&ata$e

r%spunsuri celulare

Etap% mem4ranar%

7/21/2019 Transfer Info Membranara

21/38

ransmiterea de in&ormaiiprin +ormoni polipeptidici

5"#!s

-Etap% mem4ranar%

-!E#E"!I-7 domenii transmem4ranare cu 22-2@ '' +idro&o4i-4ucla dintre $onele A *B / dom #-terminal

-cuplarea cu proteinele 5-acti0are prin cuplare cu Lig

-sitc+ 5D"*5"-acti0itate 5"a$ica-acti0ea$% proteina 5

-!egiuni de cuplare cuFGproteine 5s, 5iG"DH acronim ? prot . interact #I#:

Gpost s6naptic densit6 proteinGdisc large tumor suppresorGH-1

G:2*:?- tiro$in>ina$e . protooncogeneG!'M" . protmodactrecG'rr .arrestinG5! . " asociat% rec

GD . situs de dimeri$are

Etap% mem4ranar%

7/21/2019 Transfer Info Membranara

22/38

ransmiterea de in&ormaiiprin +ormoni polipeptidici

-Etap% mem4ranar%

-"roteine 5-protein% trimeric%-capacitate de legare a 5"-acti0itate 5"-a$ic% intrinsec%

-ipuriFG5s . acionea$% pe '# . sinte$a 'M"cG5i . in+i4% sinte$a 'M"cG5*11 . acti0ea$% "L#J "I"2 J "I"?/D'5G512*1? . semnali$are !+o 5"ase

7/21/2019 Transfer Info Membranara

23/38

7/21/2019 Transfer Info Membranara

24/38

7/21/2019 Transfer Info Membranara

25/38

' ti i %i t 4 li

7/21/2019 Transfer Info Membranara

26/38

E'"' #I"L'M'I#K - 'M"c

ransmiterea semnalului prin+ormoni polipeptidici

-sinte$a 'M"c-producerea r%spunsului celular-inacti0area semnalului

'cti0area unei c%i meta4olice

'cti0area transcripiei genice . sinte$% proteine

'cti0area unor canale ionice

- Etap% citoplasmatic%

7/21/2019 Transfer Info Membranara

27/38

R # U?UNI I RE9LAORII-In+i4% su#

-cuplarea 'M"c . deta area de su#

C # U?UNI I CAALIICE-acti0ea$% #!E prin &os&orilare

CRE # cAM> res'nsie elemen"-situl de cuplare a prot #!E pe 'DN

CRE? # CRE-%in,ing 'r"ein-&actor de transcrip ie acti0at de "'

-Etap% citoplasmatic%

- E&ectele 'M"c

7/21/2019 Transfer Info Membranara

28/38

E&ectele 'M"c

i 'M" 'cti0area unei c%i meta4olice

7/21/2019 Transfer Info Membranara

29/38

ransmiterea semnalului prin+ormoni polipeptidici

-sinte$a 'M"c-producerea r%spunsului celular-inacti0area semnalului

'cti0area unei c%i meta4olice

'cti0area transcripiei genice . sinte$% proteine

'cti0area unor canale ionice

/ inacti0area

5licogen sinteta$ei

stress

!ec & musculare

Etapa transmmem4r

E'"' #I"L'M'I#K - 'M"c

adrenalin%

7/21/2019 Transfer Info Membranara

30/38

7/21/2019 Transfer Info Membranara

31/38

i t # i I"?

7/21/2019 Transfer Info Membranara

32/38

ransmiterea semnalului prin+ormoni polipeptidici

-sinte$a #a Ci I"?-producerea r%spunsului celular-inacti0area semnalului

!eceptori cuplai cu proteina 5

intracelular, #a

- pompa de Na/-#a2/- pompare n !E!

- pompare intramitocondrial%

E'"' #I"L'M'I#K . #a / I"?

os&olipa$a #-4eta

7/21/2019 Transfer Info Membranara

33/38

ransmiterea semnalului prin+ormoni polipeptidici

E'"' #I"L'M'I#K . #a / I"?

7/21/2019 Transfer Info Membranara

34/38

7/21/2019 Transfer Info Membranara

35/38

ransmiterea de in&ormaiiprin +ormoni liposolu4ili

7/21/2019 Transfer Info Membranara

36/38

ransmiterea de in&ormaiiprin +ormoni steroi$i

7/21/2019 Transfer Info Membranara

37/38

ransmiterea de in&ormaiiprin +ormoni tiroidieni

7/21/2019 Transfer Info Membranara

38/38